So the error is fairly clear in that its indicating that something is preventing you from actually making the connection. Could it be that there is a firewall in between you and the SQL instance. Or, as indicated in the error, the settings do not allow for remote connections.
Since you indicated that it was working before, either someone made a change to the configuration in SQL, or the traffic is not making it to your SQL server.
Have you lost connection to this server? can you PING it? Is this server hosted by your or a provider?
JorgeM
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