Nucleus Detection

[sathya8819]

Hi,

I am trying to implement a project where I need to detect something. I have the digitized form of a human cell and I need to detect regions separately in the image. This part is over. The next part is where I have to detect overlapping regions. Another part is to detect the nucleus of these cells. Can someone help me with the code?

[dickersonka]

You have to show us what you got before we can help you

[mikiurban]

Yikes, this is not a very easy task. Getting the image is easy, identifying pieces of an image is not. Unless you have a very long time to dedicate, I would not attempt this from scratch. If you do, I would research "Visual Pattern Recognition" (or "Image Pattern Recognition", same thing) and "Edge Detection". Both are very difficult topics.

If you want a head start, there are some commercial products that might help you, such as "Mathwork's Image Processing Toolkit" (http://www.mathworks.com/products/image/demos.html?file=/products/demos/shipping/images/ipexcell.html)

For comparison, consider how complex Optical Character Recognition is. You have a fixed alphabet, numbers and punctuation, only two colors you have to worry about, and it is still not an exact science after 20 years of development.

[sathya8819]

i am attaching the code here for reference. this is what i have done so far and this works perfectly fine. I dont know how to proceed after this

Main Class :

AForge.Imaging.Filters.Grayscale gs = new AForge.Imaging.Filters.Grayscale(0.2125, 0.7154, 0.0721);
Bitmap b = (Bitmap)pictureBox1.Image.Clone();
b = gs.Apply(b);
BitmapData bd = b.LockBits(new Rectangle(0, 0, b.Width, b.Height), ImageLockMode.ReadWrite, PixelFormat.Format8bppIndexed);
int stride;
stride = bd.Stride;
System.IntPtr scan0 = bd.Scan0;
int label = 2;
int[,] I = new int[bd.Width,bd.Height];
exec ex1 = new exec();
b = ex1.execute(bd, scan0, I, label, stride,b);
b.UnlockBits(bd);
pictureBox2.Image = b;
b.Save(@"c:\users\pashok\b.jpeg");
pictureBox2.Visible = true;

Exec Class

int i, j;
floodclass fc = new floodclass();

unsafe public Bitmap execute(BitmapData bd, System.IntPtr scan0,int[,] I,int label,int stride,Bitmap b)
{

byte* p = (byte*)(void*)scan0;
for (i = 1; i < bd.Height; i++)
{
for (j = 1; j < bd.Width; j++)
{
if (p[i * bd.Width + j] >= 242)
p[i * bd.Width + j] = 255;

if (p[i * bd.Width + j] != 255)
I[j, i] = 1;
else
I[j, i] = 0;
}
}
for (i = 1; i < bd.Height; i++)
{
for (j = 1; j < bd.Width; j++)
{
if (I[j, i] == 1)
{
I = fc.flood(p, j, i, label, bd, I);
++label;
}
}

}
for (i = 1; i < bd.Height; i++)
{
for (j = 1; j < bd.Width; j++)
{
p[i * stride + j] = (byte)(I[j, i] * 20);
}

}
return b;
}

Flood Class

unsafe public int[,] flood(byte* p, int x, int y, int label, BitmapData bd, int[,] I)
{
Stack s = new Stack();
Point poi = new Point(x, y);
Point temp = new Point();
s.Push(poi);
while (s.Count != 0)
{
Point pp = (Point)s.Pop();
if (pp.X < bd.Width && pp.Y < bd.Height && I[pp.X, pp.Y] == 1)
{
I[pp.X, pp.Y] = label;
temp.X = pp.X - 1;
temp.Y = pp.Y;
s.Push(temp);

temp.X = pp.X + 1;
temp.Y = pp.Y;
s.Push(temp);

temp.X = pp.X;
temp.Y = pp.Y - 1;
s.Push(temp);

temp.X = pp.X;
temp.Y = pp.Y + 1;
s.Push(temp);

temp.X = pp.X - 1;
temp.Y = pp.Y - 1;
s.Push(temp);

temp.X = pp.X - 1;
temp.Y = pp.Y + 1;
s.Push(temp);

temp.X = pp.X + 1;
temp.Y = pp.Y - 1;
s.Push(temp);

temp.X = pp.X + 1;
temp.Y = pp.Y + 1;
s.Push(temp);

}
}
return I;
}

This works perfectly. But it doesnt detect overlapping regions. As in, overlapping regions are counted as a single region. How can I separate them