On line 21 of this code where I have self.greetWelcome() . I'll love for that function to be called after the GUI is loaded. What event do I have to listen for and on what object to call it after the GUI is loaded. As of now, it works before the GUI loads up.

#!/usr/bin/env python
# 
# @author: Joseph Rex
# @website: http://josephrex.me
# @repository: http://github.com/bl4ckdu5t/registron
# # #
import sys, webbrowser
from PyQt4 import QtGui, QtCore
from ui_registron import Ui_MainWindow
try:
    import pyttsx
except ImportError:
    raise ImportError, "pyttsx module is required for speech features of registron"

class Main(QtGui.QMainWindow):
    """Main class for registron"""
    def __init__(self):
        QtGui.QMainWindow.__init__(self)
        self.ui = Ui_MainWindow()
        self.ui.setupUi(self)
        self.greetWelcome()
        self.ui.proceedBtn.clicked.connect(self.checkCampusID)

        #Menu Actions
        self.ui.actionQuit.triggered.connect(self.close)
        self.ui.actionDocumentation.triggered.connect(self.openGitPage)
        # self.ui.actionAbout.triggered.connect(self.aboutProgram)
        # self.ui.actionCredits.triggered.connect(self.showCredits)
        # self.ui.actionNewWindow.triggered.connect(self.cloneWindow)
        # self.ui.actionSignIn.triggered.connect(self.adminAuth)
    def greetWelcome(self):
        function.talk("Welcome to Registron")
    def checkCampusID(self):
        campusID = self.ui.matricInput.toPlainText()
    def openGitPage(self):
        webbrowser.open('https://github.com/bl4ckdu5t/registron')


class programFunctions:
    """Core functions for registron"""
    def talk(self, speech):
        """Uses pyttsx module for speech"""
        engine = pyttsx.init()
        engine.say(speech)
        engine.runAndWait()

function = programFunctions()


if __name__ == '__main__':  
    app = QtGui.QApplication(sys.argv)
    window = Main()

    window.show()
    sys.exit(app.exec_())

Try this ...

#!/usr/bin/env python
#
# @author: Joseph Rex
# @website: http://josephrex.me
# @repository: http://github.com/bl4ckdu5t/registron
# # #
import sys, webbrowser
from PyQt4 import QtGui, QtCore
from ui_registron import Ui_MainWindow
try:
    import pyttsx
except ImportError:
    raise ImportError, "pyttsx module is required for speech features of registron"

class Main(QtGui.QMainWindow):
    """Main class for registron"""
    def __init__(self):
        QtGui.QMainWindow.__init__(self)
        self.ui = Ui_MainWindow()
        self.ui.setupUi(self)
        #self.greetWelcome()
        self.ui.proceedBtn.clicked.connect(self.checkCampusID)

        #Menu Actions
        self.ui.actionQuit.triggered.connect(self.close)
        self.ui.actionDocumentation.triggered.connect(self.openGitPage)
        # self.ui.actionAbout.triggered.connect(self.aboutProgram)
        # self.ui.actionCredits.triggered.connect(self.showCredits)
        # self.ui.actionNewWindow.triggered.connect(self.cloneWindow)
        # self.ui.actionSignIn.triggered.connect(self.adminAuth)
    def greetWelcome(self):
        function.talk("Welcome to Registron")
    def checkCampusID(self):
        campusID = self.ui.matricInput.toPlainText()
    def openGitPage(self):
        webbrowser.open('https://github.com/bl4ckdu5t/registron')


class programFunctions:
    """Core functions for registron"""
    def talk(self, speech):
        """Uses pyttsx module for speech"""
        engine = pyttsx.init()
        engine.say(speech)
        engine.runAndWait()

function = programFunctions()


if __name__ == '__main__':
    app = QtGui.QApplication(sys.argv)
    window = Main()

    window.show()

    window.greetWelcome()

    sys.exit(app.exec_())
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