I have a major concern about this. Recenlt i am doing a sequenclick game. in 16 boxes, when start, there are randomly 16 different numbers coming up.

is it possible?

Yes.

I'm assuming you're using C++/CLI with Winforms? Place your images in a List<Bitmap> and make a "surrogate" array of integers from 0 to 15. Use a Fisher-Yates shuffle on that array of integers. Display the items of the list in the order that is defined by the shuffled integer array.

See where you can get with that, and post back with any specific syntactical issues that you have.