Hi..i m new here..i want to ask some little help..how can i save the listbox items into binary file? The items should write at specific location which is offset 0x2E (item1), 0x9A (item2) and 0x106 (item3)..each offset separate by 108 length..

Do not think about ListBoxItems. Think about data.
What you should be doing is storing the data in the file.
Since you are specifying the file layout, I'd recommend creating a repository class for loading/storing the data. Look at System.IO for classes to do the low level io.