I need help playing audio from mysql blob. I know how to save audio file as blob but I don't know how to play it in vb.net. Is there a way to play audio from mysql blob without saving it first as a file? If so, I need sample codes, thanks.

You will have to store the audio file's raw binary in the database.

Here is a nice article that does this in ASP.NET (VB.NET code behind)