I know the codes not the best well written but the only problem I'm having(besides the interpreting of the macro commands, the one here is just a base for me to work on) is how the UpdateLabels.start() method returns:

Traceback (most recent call last):
  File "C:\Users\Erik\Desktop\Stuff from last Night\MouseMacro.py", line 77, in <module>
    main()
  File "C:\Users\Erik\Desktop\Stuff from last Night\MouseMacro.py", line 75, in main
    UpdateLabels().start()
  File "C:\Python30\lib\threading.py", line 446, in start
    if not self._initialized:
AttributeError: 'UpdateLabels' object has no attribute '_initialized'

This is my code:

#Python 3k
from tkinter import *
from threading import Thread

import mouse#Third Party found at http://www.python-forum.org/pythonforum/viewtopic.php?f=2&t=8976

XPOS = 0
YPOS = 0

class UpdateLabels(Thread):#activates in main...did not work?
    def __init__(self):
        global XPOS
        global YPOS
    def _initialize(self):
        run()
    def run(self):
        XPOS = mouse.getpos()[0]
        YPOS = mouse.getpos()[1]
def Help():
    messagebox._show(title="Help", message="Do This...")#add help message

def Load_Macro():#Add Lots of Error Checking here!!!!!!!!!!!!!(Make sure file exitsts!)
    f = open(filedialog.askopenfilename(), 'r')
    data = f.read()
    f.close()
    txt_Macro.delete(1.0, END)
    txt_Macro.insert(END, data)


def Interpret(text):#NEED TO FIX
    text = text.split(';')
    for i in text:
        com = i.split()
        if com[0] == 'MOV':
            #parse for move command
            b = com.split()
            mouse.move(int(b[0]), int(b[1]))
        elif com[0] == 'CLK':
            mouse.click()
        elif com[0] == 'SLD':
            #parse for where to move and speed 'slow' or 'fast'
            b = com.split()
            mouse.slide(int(b[0]), int(b[1]), b[2])
        elif com[0] == 'HOLD':
            mouse.hold()
        elif com[0] == 'RCLK':
            mouse.rightclick()
        elif com[0] == 'RHOLD':
            mouse.righthold()
        else:
            return False#returns an ERROR if messup
def start_macro():
    Interpret(txt_Macro.get(1.0, END))
    print(txt_Macro.get(1.0, END))

root = Tk()

#Use these to update mouse positions
lbl_mouse_xpos = Label(root, text="Mouse X Pos: "+str(XPOS))
lbl_mouse_ypos = Label(root, text="Mouse Y Pos: "+str(YPOS))

txt_Macro = Text(root, height=1)#Use this to get macro text

cmd_start_macro = Button(root, text="Start", command=start_macro)
cmd_help = Button(root, text="Help", command=Help)
cmd_load_macro = Button(root, text="Load Macro", command=Load_Macro)

def main():
    lbl_mouse_xpos.pack()
    lbl_mouse_ypos.pack()
    txt_Macro.pack()
    cmd_start_macro.pack()
    cmd_help.pack()
    cmd_load_macro.pack()
    UpdateLabels().start()
    root.mainloop()
main()

Any help with the error message and the threading module would be greatly apreciated.

I quote the python 3.0 documentation: "If the subclass overrides the constructor, it must make sure to invoke the base class constructor (Thread.__init__()) before doing anything else to the thread." So I think you should start your class like this

class UpdateLabels(Thread):#activates in main...did not work?
    def __init__(self):
        Thread.__init__(self)
        global XPOS
        global YPOS

Also, I don't understand your method _initialize . I don't think you need it.

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