A question about biopython

[polopaul]

for seq_record in SeqIO.parse(open("mm.fa"), "fasta") :

I am using the above code to read fasta files. But when the number of sequences record exceed about 30000, the python return this error:

Traceback (most recent call last):
File "C:\Python26\Neutral\AICMM.py", line 28, in <module>
if (seq_record.seq[count] == "A") :
File "C:\Python26\lib\site-packages\Bio\Seq.py", line 157, in __getitem__
return self._data[index]
IndexError: string index out of range

Anyone who can help me with this?
Thank you very much

[Tech B]

I haven't worked with fasta files nor do I know what they are.
But have you tried putting it in a try, except statement?

try:
  for seq_record in SeqIO.parse(open("mm.fa"), "fasta") :
    do something
except IndexError:
  pass #or break

Or maybe append the values you get from the file to a list, and work with the items of the list instead?

[woooee]

if (seq_record.seq[count] == "A") :

There is apparently a 32K limit on the number of records (short integer, signed). You will have to come up with a way to split the data up or use another method to read.