def askopenfilename(self):
        filename= tkFileDialog.askopenfilename(initialdir='C:/..',title='Select File' , filetypes=[('Bitmap Images', '*.bmp'),('Png Images', '*.png'),('Gif Images', '*.gif'),("Jpeg","*.jpg"),("All files", "*")])
        self.entryVariable.set(filename)
        if filename == None:
            return open(filename,'rb') 
            self.entry.update(filename)
        self.image = Image.open(filename)
        self.img = ImageTk.PhotoImage(self.image)


        self.canvas.config(width=400, height=300)
        self.lab.img = self.img
        self.lab.pack()
        self.canvas.create_image(1,1, anchor=NW, image=self.img)
        self.scrollbar_vert = Scrollbar(self.canvas)
        self.scrollbar_vert.pack(side=RIGHT, fill=Y)
        self.scrollbar_hor = Scrollbar(self.canvas)
        self.scrollbar_hor.config(orient=HORIZONTAL)
        self.scrollbar_hor.pack(side=BOTTOM, fill=X)
        self.canvas.config(yscrollcommand=self.scrollbar_vert.set)
        self.canvas.config(xscrollcommand=self.scrollbar_hor.set)
        self.canvas.config(scrollregion=self.canvas.bbox(ALL))
        self.scrollbar_vert.config(command=self.canvas.yview)
        self.scrollbar_hor.config(command=self.canvas.xview)




    def encode_files(self):
        image = self.lab.img
        data_in = self.data_entry
        data = data_in.read()
        encoded = LSB.encode(image, data)
        image.save('C:\\Python27\\self.image_new.png')


    self.canvas.config(xscrollcommand=self.scrollbar_hor.set)
    self.canvas.config(scrollregion=self.canvas.bbox(ALL))
    self.scrollbar_vert.config(command=self.canvas.yview)
    self.scrollbar_hor.config(command=self.canvas.xview)




def encode_files(self):
    image = self.lab.img
    data_in = self.data_entry
    data = data_in.read()
    encoded = LSB.encode(image, data)
    image.save('C:\\Python27\\self.image_new.png')

What does it do differently than what you expect? A simple example with an explanation of what you want will get faster and better results

Note that encode_files is declared twice and there are indentation errors in the code.

Somewhat goofy partial code.
Could your problems be with the steganography processing?
Did you try to put a normal image to test?

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