Sure you can. Simply create new DGVcomboBox column and add or insert it to dgv columns.

DataGridViewComboBoxColumn cmbcolumn = new DataGridViewComboBoxColumn(); 
{
    cmbcolumn.Name = "cmbColumn";
    cmbcolumn.HeaderText = "combobox column"; 
    cmbcolumn.Items.AddRange(new string[] { "aa", "ac", "aacc" }); //items in comboBox
}
dataGridView1.Columns.Insert(1, cmbcolumn); //inserting comboBox into 2nd columns (colimn at index 1)
This question has already been answered. Start a new discussion instead.